Vapor inhalation of cannabidiol (CBD) in rats.

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RATIONALE:

Cannabidiol (CBD), a compound discovered in lots of strains of the Hashish genus, is more and more out there in e-cigarette liquids in addition to different merchandise. CBD use has been promoted for quite a few purported advantages which haven’t been rigorously assessed in preclinical research.

OBJECTIVE:

To additional validate an inhalation mannequin to evaluate CBD results within the rat. The first purpose was to find out plasma CBD ranges after vapor inhalation and examine that with the degrees noticed after injection. Secondary objectives had been to find out if hypothermia is produced in male Sprague-Dawley rats and if CBD impacts nociception measured by the nice and cozy water tail-withdrawal assay.

METHODS:

Blood samples had been collected from rats uncovered for 30 min to vapor generated by an e-cigarette system utilizing CBD (100, 400 mg/mL within the propylene glycol car). Separate experiments assessed the physique temperature response to CBD together with nicotine (30 mg/mL) and the anti-nociceptive response to CBD.

RESULTS:

Vapor inhalation of CBD produced concentration-related plasma CBD ranges in female and male Wistar rats that had been inside the vary of ranges produced by 10 or 30 mg/kg, CBD, i.p. Dose-related hypothermia was produced by CBD in male Sprague-Dawley rats, and nicotine (30 mg/mL) inhalation enhanced this impact. CBD inhalation had no impact on anti-nociception alone or together with Δ9-tetrahydrocannabinol inhalation.

CONCLUSIONS:

The vapor-inhalation method is an appropriate pre-clinical mannequin for the investigation of the results of inhaled CBD. This route of administration produces hypothermia in rats, whereas i.p. injection doesn’t, at comparable plasma CBD ranges.

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